A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
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چکیده
منابع مشابه
A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swi...
متن کاملRapid Detection of Foot-and-Mouth Disease Virus by Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP)
The aim of this study was to produce a sensitive, simple and rapid diagnostic method for the detection of foot-and-mouth disease virus (FMDV) in suspected cases of FMD, a one-step single-tube method of reverse transcription loop-mediated isothermal polymerase amplification (RT-LAMP). A set of six common primers was designed to target the highly conserved region of the RNA polymerase 3Dpol gene ...
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Successful disease management requires a rapid and sensitive diagnosis method that can recognize early infection even before the manifestation of its clinical signs. The only available field diagnostic tests for foot-and-mouth disease (FMD) are lateral flow devices, commonly known as chromatographic strips. Low sensitivity and inability to detect FMD virus (FMDV) at the serotype level are limit...
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The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a d...
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ژورنال
عنوان ژورنال: Journal of Veterinary Science
سال: 2014
ISSN: 1229-845X,1976-555X
DOI: 10.4142/jvs.2014.15.3.423